GENCODE PCR-Seq Batch XIV. GENCODE PCR-Seq Batch XIV

As part of the ENCODE consortium the GENCODE project is producing a reference gene set through manual and automated gene prediction. Selected transcript models are verified experimentally by RT-PCR amplification of at least one of their unique splice junctions followed by sequencing. The experiment targets are manually annotated transcripts with novel or putative status, non-pseudogene biotype and unique splice junctions not validated previously. For Batch XIV 271 splice junctions from GENCODE16 (released April 2013) were chosen for experimental verification.