Additional file 9 of FAM20B-catalyzed glycosaminoglycans control murine tooth number by restricting FGFR2b signaling

Additional file 9: Figures S9A-S9B. Fig. S9A-[GAGs did not show synergistic effects on FGF10-FGFR2b signaling]. Fig. S9B-[GAGs did not show inhibitory effects on FGF10-FGFR2b signaling]. HS and CS did not show significant synergistic or inhibitory effects on FGF10-FGFR2b signaling in BaF3 cells. A BaF3 cells expressing FGFR2b were cultured in RPMI 1640 media supplemented with 1000 pM FGF10 and 0–5 ng/ml GAGs (HS/HS2S/HS6S/CSA/heparin) for 45 h. Heparin (positive control) showed significant synergistic effects on FGFR2b signaling (P < 0.001), while HS/HS2S/HS6S/CSA groups did not show any significant synergistic effects (P > 0.05). B BaF3-FGFR2b cells were cultured in RPMI 1640 media supplemented with 1000 pM FGF10 and 1.5 μg/ml heparin to boost the baseline of FGFR2b signaling activity (indicated by cell viability OD value). HS/CSA/CSC/CSE/LMW heparin (0–5 ng/ml) supplemented to the culture media did not show any inhibitory effects on FGFR2b signaling (P > 0.05). Two-way ANOVA was used to evaluate the differences among groups.