File S1 - Novel Inhibitor Cystine Knot Peptides from Momordica charantia

Table S1 & S2, Figure S1 & S2. Table S1 Sequence fragments and sequences from the enzymatic digestion of the native and alkylated peptides from M. charantia. Figure S1 Quantification of Ia, IIa and MCh-1 observed during reductive unfolding and oxidative refolding in different time courses. (A) Reduced MCh-1 with 1 mM GSH. (B) Reduced MCh-1 without GSH. (C) IIa intermediate. Figure S2 TOCSY and NOESY spectra of MCh-1. (A) The TOCSY amide region and spin systems of MCh-1. (B) The NOESY fingerprint region and sequential connectivity via Hα-HN of MCh-1. Table S2 NMR and refinement statistics of MCh-1 and MCh-2. (DOC)