Changes in the biologica function of necroptotic, peripheral blood mononuclear cells under irradiation in vitro

The secretome of y-irradiated peripheral blood mononuclear cells (PBMC) is well known for its pleiotropic effects on tissue protection and regeneration. Irradiation of PBMC lead to necroptosis of a certain amount of PBMC in a cell-sorter analysis. The aim of this study was to investiage the trancriptomic changes of PBMC and PBMC subsets (CD4, CD8, CD19, CD56, CD14) in response to y-irradiation In total 72 samples obtained from four healthy donors were analysed (0h= cells immediately after purification, 24h_0Gy = cells cultivated for 24 hours without prior radiation; 24h_60Gy = cell cultivated for 24 hours after irradiation with 60Gy). At each time point six different cell populations were analysed (unseparated PBMC, and separated PBMC subsets: CD4, CD8, CD14; CD19; CD56).