H3K27ac HiChIP analysis in GI-MEN DOX-ASCL1 cells

H3K27ac HiChIP analysis was performed in GI-MEN DOX-ASCL1 cells to analyze active chromatin-chromatin interactions in GI-MEN DOX-ASCL1 cells. Overall design: Cells were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP), tagmented with Tn5 and analyzed by DNBseq. HiChIP was performed using an antibody against H3K27ac Antibody (ab4729).