CAGE-Seq of EHV-1 transcriptome of a mixed infection. The virus isolate is the Mc13, which was propgataed on Rk-13 cells.

We applied Cap Analysis of Gene Expression (CAGE) to uncover the TSS distribution patterns at the examined genomic regions in EHV-1 using three biological replicates. The CAGE™ Preparation Kit (DNAFORM, Japan) was applied. The libraries were sequenced on a MiSeq instrument with v3 (150 cycles) and v2 (300 cycles) chemistries (Illumina). The RNA-samples derived from a mixed time points of EHV-1 viral infection (isolate Mc13, originated from Szilvasvarad, Marocpuszta, Szilvasvarad). EHV-1 viruses were propagated on Rabbit Kidney -13 cell line.