Single-base resolution methylome of 5-methylcytosine in mammalian transcriptome
收藏NIAID Data Ecosystem2026-03-10 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP065906
下载链接
链接失效反馈官方服务：
资源简介：
mRNAs of human and mouse cell lines were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement. mRNAs were fragmented to ~100 nt and treated by Sodium bisulfite solution (pH 5.1) containing Hydroquinone. Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA) and performed sequencing on HiSeq2000/3000 (Illumina) in pair-end mode, creating reads with a length of 101 bp. Sequencing chemistry v2 (Illumina) was used and samples were multiplexed in two samples per lane. Overall design: Explore the single-base resolution methylome of 5-Methylcytosine in mammalian transcriptome
创建时间：
2018-10-23
