Immune crosstalk between pancreas, gut and liver through lymph node sharing [scRNA-seq]

To investigate tissue of origin effects on migratory dendritic cell (DC) gene expression in shared lymph nodes we performed bulk RNAseq of DCs from each tissue (liver, pancreas, duodenum). We then performed single cell sequencing of the DCs within the draining lymph nodes. scRNAseq of FACs purified migratory dendritic cells from liver, pancreas and duodenum (n=4 each tissue, each tissue is 2 mice pooled) and single cell sequencing of YFP+ cells sorted from CD11c-YFP pancreatic-duodenal lymph nodes (n=3 each lymph node, each lymph node is 10 mice pooled).