Effect of SMARCA4 knockdown on senescent IMR90 ER:RAS cells. Effect of SMARCA4 knockdown on senescent IMR90 ER:RAS cells

We report the analysis of RNA sequencing aimed at understanding the effects induced by the knockdown of various target genes on senescent cells. These genes were initially identified through a genetic screening process designed to locate regulators responsible for upregulating SASP. Specifically, we focus on exploring the repercussions of SMARCA4 knockdown, a constituent of the SWI/SNF complex subunit, on the SASP and immune responses within senescent cells. The findings will help characterise the role of SMARCA4 inhibition on senescence surveillance by Natural Killer cells. Overall design: IMR90 ER:RAS cells were transfected with siRNAs against target genes and non-targeting scramble controls (by reverse transfection) one day prior to senescence induction. Oncogene-induced senescence was induced following the administration of 4OHT (tamoxifin) treatment. RNA from the cells was extracted seven days following senescence induction.