Supplementary Material for: DNA methylation of the ABCA1 and ABCG1 genes contribute to stroke risk

Introduction: Stroke is a major cause of death in China. The epigenetic factors, especially the methylation of ABCA1/G1 genes implicated in cholesterol regulation, are being examined to comprehend their association with stroke.
Methods: In this nested case-control study, we examined data from the North-west China Cohort (CNC-NX) initial phase, involving 53 pairs of stroke cases and controls. All participants were adults aged ≥18 years. We examined the conditional logistic regression models were used to determine the risk ratio (OR) and 95% confidence interval (95% CI) for stroke occurrence.
Results: CpG_10.11.12.13 methylation levels in ABCA1 were associated with stroke risk (OR 0.93, 95% CI: 0.87–0.99), and dynamic changes in CpG_19.20 methylation levels in ABCG1 were associated with stroke risk (OR 1.62, 95% CI: 1.11–2.37). In dynamic methylation, high methylation levels of CpG_19.20 in ABCG1 were associated with a 5.10 times higher risk of stroke compared to low methylation levels (OR 5.10, 95% CI: 1.60–16.30).
Conclusions: In a rural population in North-West China, the hypomethylation status of ABCA1 and ABCG1 genes was strongly associated with the incidence of stroke. Significant correlations between CpG_19.20 methylation levels change in ABCG1 and stroke risk.