A zebrafish model to elucidate the impact of host genes on the microbiota

Every host species and organism provide a unique environmental niche contributing to the overall diversity of microbial ecosystems from the intestine of an animal to the oceans and forests of our planet. The study of host-microbiota interactions has long focused on the well-established effects the microbiota has on its host. In contrast, little focus has been allocated to the role of the host in these intricate interactions. However, understanding the role of the host may well be an essential key to understanding the complexity of the relationship between the host and its microbiota. In this study, we present a model in which the effects of host genes on the microbiota can be elucidated and how such genetic effects may shape host-associated microbiota. We demonstrate a hologenomic approach implementing the CRISPR/Cas system in the zebrafish model to combine the effects of a host gene with 16S metabarcoding and metabolomics data. We show that knocking out the gene coding for the rate-lim..., , , # A zebrafish model to elucidate the impact of host genes on the microbiota

The dataset is composed of metadata, files and tables related to 16S V3-V4 sequencing and processing of zebrafish related samples, tank water samples and controls. The fish are derived from a CRISPR gene editing protocol for knocking out a pigmentation gene (*tyr*).

## Description of the data and file structure

The data is composed of ASV tables and taxonomy tables generated from processing 16S V3-V4 amplicons and associated metadata.

1. Metadata
2. ASV table and taxonomy table generated from dada2 including a fasta file of the ASVs (
3. ASV table and taxonomy table generated from decontamination of (1.) using the R package decontam (
4. ASV table and taxonomy table generated from ASV curation of (2.) with the R package LULU (

File list:
Metadata_complete.csv
ASV_table_dada2.tsv
ASV_taxonomy_table_dada2.tsv
ASVs.fa
ASV_taxonomy_table_decontam.tsv
ASV_table_decontam.tsv
LULU_curated_ASV_table.txt
LULU_...