Analysis of the BadR regulon in Borrelia burgdorferi

To investigate the genes regulated by BadR in Borrelia burgdorferi, a badR knockout was generated by homologous recombination. The badR mutant and wild-type strains were cultivated in vitro under conditions mimicking the unfed tick and mammalian host. Under mammalian host conditions, cells were collected at two growth phases to determine if BadR regulates genes in a growth-phase dependent manner.