Single-Cell RNA Sequencing of Brain Endothelial Cells in 12-Month-Old Male 5XFAD and Wild-Type Mice

This study investigates Alzheimer's Disease-related transcriptomic changes in brain endothelial cells of 5XFAD mice on the C57BL/6J background using single-cell RNA sequencing. Brains from male hemizygous 5XFAD (n=3) and wild-type control mice (n=3) aged 12 months were used. Brain tissue (excluding olfactory bulbs and cerebellum) was dissociated into a single cell suspension, and Fluorescence Activated Cell Sorting (FACS) was used to isolate CD31+, CD45- cells. Cells were encapsulated and sequenced using the 10x Genomics platform. Libraries were sequenced on an Illumina NovaSeq XP, targeting ~50,000 reads per cell. In our analysis, raw data were aligned to the mouse (GRCm39)-2024-A reference genome. FASTQs are named: 13389-RE-1 (Wild-type 1), 13389-RE-2 (Wild-type 2), 13389-RE-3 (Wild-type 3), 13389-RE-4 (5XFAD 1), 13389-RE-5 (5XFAD 2), 13389-RE-6 (5XFAD 3).