Gene expression profiling of CA10 and NHD13 murine AML

We have compared the gene expression profiles of leukemic tissues in two different mouse models of AML, CALM-AF10 and NHD13, to clincally healthy transgenic and wildtype hematopoetic tissue to identify genes and pathways that can collaborate with these oncogenic fusion proteins to promote leukemic transformation. CA10 and NHD13 transgenic mice were sacrificed when clinical signs of leukemia manifested (mice were hunched, skinny, or had an abnormal complete blood count). For comparison, we harvested age matched bone marrow, thymi, and spleen from wildtype and clinically healthy transgenic tissues. Transgenic animals were considered clinically healthy if no evident signs of leukemia were present.本研究将两种急性髓系白血病（Acute Myeloid Leukemia, AML）小鼠模型（CALM-AF10与NHD13）的白血病组织基因表达谱，与临床健康的转基因及野生型造血组织进行比对，旨在筛选可与这些致癌融合蛋白协同促进白血病转化的基因与信号通路。当CALM-AF10（简称CA10）与NHD13转基因小鼠出现白血病临床表征（弓背、消瘦或全血细胞计数异常）时，对其实施安乐死。为开展对照实验，我们从野生型及临床健康的转基因小鼠体内采集年龄匹配的骨髓、胸腺与脾脏组织。未出现明确白血病症状的转基因动物，将被认定为临床健康个体。