Rational selection of reverse phase columns for high throughput LC-MS lipidomics.

Human blood plasma was chosen as a representative complex natural lipidome with large variance of lipid classes, species and lipid concentrations. Lipids were separated by RPLC on five different reverse phase columns with different types of stationary phase particles, size and chemistry. High mass accuracy MS analysis and data-dependent MS/MS analysis were performed using a Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer to identify individual lipid molecular species. This workflow was applied to evaluate the separation capability of each column and to identify the lipidomics profile in highly complex biological samples. As a result, we report more than 600 lipid species covering 18 lipid classes in human blood plasma and provide suggestions to the selection of the appropriate reverse phase column for the analysis of specific lipidomes.

In each folder are reported the 6 replicates (3 in positive, 3 in negative ion mode) for the 5 column used:
- Accucore C18 (2.1 x 150 mm, 2.6 µm)
- HypersilGOLD C18 (2.1 x 150 mm, 1.9 µm)
- Accucore C30 (2.1 x 150 mm, 2.6 µm)
- Acclaim C30 (2.1 x 150 mm, 1.9 µm)
- Acclaim C30 (2.1 x 250 mm, 3.0 µm)

人类血液血浆被选作具有脂质类别、物种和脂质浓度广泛变异的代表性复杂天然脂质ome。通过使用五种不同类型固定相粒子、尺寸和化学性质的逆相液相色谱（RPLC）柱对脂质进行分离。采用Q Exactive HF 混合四极杆-轨道阱质谱仪进行高质量精度MS分析和数据依赖MS/MS分析，以鉴定单个脂质分子物种。该工作流程被应用于评估每根柱子的分离能力，并识别高度复杂生物样本中的脂质组学特征。因此，我们报告了超过600种脂质物种，涵盖人类血液血浆中的18种脂质类别，并就选择合适的逆相柱以分析特定脂质ome提供了建议。

在每个文件夹中，均报告了用于五种不同柱子的6个重复样本（正离子模式3个，负离子模式3个）：
- Accucore C18 (2.1 x 150 mm, 2.6 µm)
- HypersilGOLD C18 (2.1 x 150 mm, 1.9 µm)
- Accucore C30 (2.1 x 150 mm, 2.6 µm)
- Acclaim C30 (2.1 x 150 mm, 1.9 µm)
- Acclaim C30 (2.1 x 250 mm, 3.0 µm)