Source Data for Figures

Source Data for Figures from "Rapid, tunable, and multiplexed detection of RNA using convective array PCR". Each dataset is presented in a separate Sheet within the Microsoft Excel file. Both 'raw' and 'normalized' datasets are provided for each Figure, wherein 'raw' corresponds to background-subtracted data (f-hat in the language of the data treatment pipeline as described in the Supplement), and 'normalized' features the full background-subtraction, baseline-subtraction, and maximum-fluorescence normalization treatments. The included datasets are: Full microarray data for the plot in Figure 2c (Omicron template)12 datasets corresponding to each of the templates tested in Figure 3b (484E, 484K, 484Q, 484STOP, 484E_1mut, 484K_1mut, 484Q_1mut, 484STOP_1mut, 484E_2mut, 484K_2mut, 484Q_2mut, 484STOP_2mut), where the name and sequence of each template is given in Table S7. Fluorescence data for Probe 1 - Probe 6 given in the dataset correspond to the probes designed against the 484K genotype (i.e., the curves shown in the Figure)8 datasets corresponding to each of the templates tested in Figure 3d (SARS-CoV-1N, SARS-CoV-1N1.1, SARS-CoV-1N1.2, SARS-CoV-1N2, SARS-CoV-2N, SARS-CoV-2N1.1, SARS-CoV-2N1.2, SARS-CoV-2N2), where the name and sequence of each template is given in Table S8. Fluorescence data are given for the SARS-CoV-1 and SARS-CoV-2 probes targeting the nucleocapsid gene for each dataset. Full microarray data for the plot in Figure 4a (the same data are used to construct the compressed plot pair in Figure 4b; Omicron template)Three full microarray datasets for each of the three compressed plot pairs in Figure 4c (HCoV-229E, MERS-CoV, SARS-CoV-1 templates)Full microarray data used to produce the compressed plot pair in Figure 4d (Delta template)6 full microarray datasets used to produce each of the compressed plot pairs in Figure 5 (SARS-CoV-2 Delta alone, HCoV-229E alone, Influenza B alone; SARS-CoV-2 Delta + HCoV-229E, SARS-CoV-2 Delta + Influenza B; SARS-CoV-2 Delta + HCoV-229E + Influenza B)