Figure S4

Figure S4: Kidney peptidergic and mechanosensory nerve markers are detected after kidney innervation commences. (A) E14.5 kidney sections immunostained for CGRP (magenta) shows that axons at this stage (TUBB3, green) which primarily associate with the vasculature (CD31, yellow) are not yet CGRP+. (B) Trpv1Cre/+;R26tdTomato/+ E16.5 and P1 kidney sections immunostained for CGRP (green), TUBB (yellow), and tdTomato (TRPV1 lineage; magenta) show that tdTomato+ sensory nerves colocalize with CGRP (arrowheads). (C) E14.5 kidney sections immunostained for substance P (SubP, magenta) shows that axons at this stage (TUBB3, green) which primarily associate with the vasculature (CD31, yellow) are not yet SubP+. (D) Wholemount immunostain of an E18.5 kidney showing PIEZO2+ (magenta) axons colocalizing with TUBB3 (green).