cAMP and mGluR1/5 dependent expression of mRNA, lncRNA, and miRNA in mouse hippocampal neurons

To identify cAMP-dependent and mGluR1/5-dependent RNAs, we treated DIV16 primary hippocampal neurons with 50uM Forskolin, 30uM DHPG, or equal volume DMSO for 30 minutes prior to RNA isolation using Trizol and Total/small RNA sequencing. We submitted samples to Scripps Florida Genomics Core for library preparation and sequencing. Overall design: Hippocampi were cultured from P0 mice for generating RNA at DIV 16. We treated DIV16 primary hippocampal neurons with 50uM Forskolin, 30uM DHPG, or equal volume DMSO for 30 minutes.