Characterization of stress-susceptible cells and gene in mouse brain via Spatial Transcriptomics

Spatial transcriptome analysis of brain sections from non-stressed controls (CON), susceptible mice (SUS) and resistant mice (RES) was performed to investigate spatial patterns of gene expression in multiple brain regions to gain new insights into stress susceptibility. Animals were subjected to 10 days of chronic social defeat stress and then phenotypes were acquired using social interaction tests.Fresh brain tissue was collected 1.5 hours after the last CSDS exposure of mice, and we obtained three coronal sections(Bregma2.0~2.1) from the left brain of Control, susceptible, resilient mouse by cryosections of mouse brains. After that Spatial transcriptome (ST) sequencing was performed.