Illumina sequencing of 3.5 kb jumping libraries on 1000 Genomes Trios for SV discovery. Illumina sequencing of 3.5 kb jumping libraries on 1000 Genomes Trios for SV discovery

Illumina sequencing of 3.5 kb jumping libraries on 1000 Genomes Trios for SV discovery. Libraries were generated following the protocol described by Hanscom and Talkowski (2014). (http://www.ncbi.nlm.nih.gov/pubmed/24789519). Targeted fragment size was 3.5 kb, targeted genome coverage was >100X, and EcoP15I restriction fragment digestion of circularized DNA results in paired-end 25 bp reads that should be reverse complemented before alignment.