Gene Expression in the Lrrc10 knockout heart

LRRC10 is a heart-specific gene required for proper cardiac function. The effects of Lrrc10 deletion on gene expression in the adult mouse heart was investigated. Lrrc10 knockout mice or wild type controls, housed in 12 hour light:12 dark, ad lib feeding and drinking conditions were sacrificed at two months of age for cardiac gene expression analysis. A two color, reference design experiment in which heart RNA from 2 Lrrc10 knockout mice was pooled and labeled with Cy5 and hybridized according to Agilent protocols against a reference pool of RNA made up from respective tissue taken from 2 month wild type mice which was labeled with Cy3.LRRC10是一种心脏特异性基因，对维持正常心脏功能不可或缺。本研究探究了Lrrc10基因敲除对成年小鼠心脏基因表达的影响。将饲养于12小时光照:12小时黑暗循环、自由进食饮水环境下的Lrrc10敲除小鼠与野生型对照小鼠，于2月龄时处死，用于心脏基因表达分析。本实验采用双色参考设计方案：将2只Lrrc10敲除小鼠的心脏RNA混合后用Cy5标记，并按照安捷伦（Agilent）实验流程，与由2月龄野生型小鼠相应组织提取的RNA混合参考池（该参考池已用Cy3标记）进行杂交。