RNA-sequencing of Chronic Phase Shift Protocol on Murine Pancreas

The objective of this study was to understand the long-term effects of a chronic phase shift protocol on the pancreatic transcriptome. A total of 144 wild-type C57Bl/6J 4-6 week old mice were housed under normal circadian conditions or disrupted using a chronic phase shift protocol whereby the light-dark cycle is advanced 8 hour every 2-3 days for 4 weeks. 12 hours prior to sacrafice mice were normalized under normal circadian conditions. Starting at ZT 0, mice were serially sacraficed at 4-hour intervals for 48 hours and the pancreas extracted. Pancreatic RNA was isolated and subjected to RNA-seq. Overall design: A total of 144 samples were collected. Males (n = 3) and females (n=3) from each timepoint were pooled prior to sequencing. A total of 6 mice were sacraficed for each condition (normal circadian and circadian disrupted) at each timepoint.