Gene expression analysis of Arabidopsis root in LBD16-GR overexpression line

Lateral roots (LRs) are formed post-embryonically and contribute to root architecture formation in vascular plants. LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16) is a key transcription factor to initiate LR formation. LBD16 functions downstream of AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19, and overexpression of LBD16 partially restores LR formation in the absence of ARF7 and ARF19. To identify downstream targets of LBD16, we engineered a transgenic line with inducible LBD16 activity by overexpressing a fusion protein of LBD16 and rat glucocorticoid receptor (GR) in arf7 arf19 mutant. Here we identified primary response genes of LBD16 from transcriptome analysis of 35Spro:LBD16:GR arf7 arf19 line. Overexpression line of LBD16-Glucocorticoid Receptor (GR) driven by CaMV35S promoter in arf7 arf19 mutant was used for LBD16 induction. We collected roots from 5-day-old seedlings of 35S:LBD16:GR arf7 arf19 line 4 hours after treatment with/without dexamethazone (DEX) and cycloheximide (CHX). Three independent sets of mRNA samples for each treatment were used for this analysis