Mechanisms of muscle degeneration in Drosophila third instar larval musculature upon Tropomodulin (Tmod) knockdown

Summary from GEO:<br/><br/>"The knockdown of Tmod, an actin-capping protein, decreases the expression of proteasomal degradation genes and increase expression of oxidative metabolism genes. It is also critical for the correct expression of larval actin isoforms."<br/> <br/>Overall design from GEO:<br/><br/>"Performed gene expression profiling analysis using data obtained from RNA-seq of 2 experimental groups (tmodRNAi vs mCherryRNAi), with 3 samples each. Each sample contains the transcriptome of 7-10 Drosophila larva muscle enriched lysates."

GEO 摘要：

Tmod（肌动蛋白帽蛋白）的敲除降低了蛋白酶体降解基因的表达，并增加了氧化代谢基因的表达。此外，对幼虫肌动蛋白同型的正确表达也至关重要。

GEO 整体设计：

通过RNA-seq技术对两组实验样本（tmodRNAi vs mCherryRNAi）的数据进行了基因表达谱分析，每组样本包含3个样本。每个样本均富含7-10天龄果蝇幼虫肌肉的转录组。