Chromatin state changes induced by targeted degradation of MLL-AF9 are phenocopied by combined DOT1L and MENIN inhibition [PRO-seq]

Using PRO-seq we examined nascent transcriptional changes and RNA Pol II pausing following MLL-AF9 degradation in MLL-AF9-HA-FKBP12 transformed hCD34+ cells (HCB1) after 15, 30, and 180 minutes Nascent gene expression changes and RNA Pol II pausing were assessed in the MLL-AF9-HA-FKBP12 transformed hCD34+ cells (HCB1) following MLL-AF9 degradation after 15, 30, and 180 minutes of 500nM dTAG-VHL treatment, compared to DMSO control