Adapting the 10x Genomics Platform for single-cell RNA-seq in yeast reveals the importance of stochastic gene expression during the lag phase

Isogenic population of yeast cells was grown in a maltose-glucose-maltose media shift schema. Time point samples were taken throughout the experiment, and were analyzed for single-cell gene expression using 10x Genomics platform. Overall design: Yeast cells experience a halt in growth after glucose-maltose shift, aka lag phase. Two samples are taken during glucose growth. Two samples are taken during lag phase in maltose. One sample comprises a mix of cells actively grown on glucose and cells actively growing in maltose. This last sample serves as a control to see if we can detect the expected population structure.