The cellular landscape of the healthy human intervertebral disc: identification of new immature cell populations and their relationship with mature populations at single cell resolution

For this study, we applied scRNA-seq to cells from surgically separated annulus fibrosus and nucleus pulposus tissues to identify A) thecellular landscapes of healthytissues, and B) characterize in detail the composition andgene regulatory networksof immature cell populations. Single cell level analysis of annulus fibrosus and nucleus pulposus tissues from human healthy (n=3, n=3) intervertebral discs (IVDs).