Genome-wide maps of chromatin state in PANC-1 cells.

To investigate functional associations between SIRT1 and CUL4B, we used ChIP-seq to analyze genome-wide SIRT1/CUL4B complex transcriptional targets. ChIP experiments were performed first in PANC-1 cells using antibodies against SIRT1 or CUL4B. Next, SIRT1- or CUL4B-associated DNA was amplified using non-biased conditions, labeled, and sequenced. Using Illumina HiSeq2000, we found lots of SIRT1- and CUL4B-specific binding peaks, respectively, representing the ChIP-seq peak. We found that strong enrichment on the promoters of selected genes involved in classical pathways. This study gave us a new understanding of the role of SIRT1/CUL4B in chromatin status and gene transcription. Examination of SIRT1 and CUL4B target genes in PANC-1 cell line