Microarray-Based Gene Expression Analysis of WT and anac060

Purpose: we performed transcriptome analysis of the wild type and anac060 mutant under glucose treatment to investigated the role of ANAC060 in glucose signalling. Methods: Agilent Arabidopsis 3.0 Oligo Microarray kit which contained nearly 40,000 features representing 28,500 genes was use to investigate of transcriptome of wild type and anac060 mutant under glucose treatment and sorbitol control. Results: By analyzing the transcriptome data, we found that ANAC060 attenuate the glucose signaling and that ANAC060 plays an important role in maintaining the Endogenous stable state of Arabidopsis thaliana in the glucose signaling pathway. The microarray analysis identified 8350 altered genes between different material and treatment combinations. Cluster analysis revealed that ANAC060 attenuates the sugar regulated gene expression. Conclusions: our results demonstrated that ANAC060 directly bind to the promoter of ABI5 and repressed the sugar induced transcription of ABI5, which contributed to render sugar insensitivity. transcriptome analysis of the 4-day wild type and anac060 mutant under glucose treatment