Gastrointestinal gd T cells reveal upregulated T-cell transcripts and signaling pathways during peanut oral immunotherapy

Oral immunotherapy (OIT) has been successful in desensitizing patients to offending food allergens, although identification of tissue-resident T cell subsets and cognate pathways leading to desensitization has been challenging. The T cells are a major T-cell subset of mucosal intraepithelial lymphocytes (IELs) and play a significant role in tissue homeostasis and repair. Studies in mouse models suggested a regulatory role of gd T cells in food allergy (FA). Also, peripheral gd T cells from patients analyzed over 24 weeks of peanut OIT were shown to undergo dynamic changes in expression profiles, implicating pathways involved in immune homeostasis. To our knowledge, the role of gd T cells in the intestinal mucosa of FA patients during immunotherapy has not been examined.  To this end, we investigated whether gd T cells in the gastrointestinal (GI) tract are modulated during peanut OIT. We hypothesized that GI-resident gd T cells in FA patients would increase during the course of peanut O..., , , # Gastrointestinal gd T cells reveal upregulated T-cell transcripts and signaling pathways during peanut oral immunotherapy

[https://doi.org/10.5061/dryad.crjdfn35n](https://doi.org/10.5061/dryad.crjdfn35n)

## Description of the data and file structure

Supplementary File 1 : Differentially expressed genes predicted in sorted gdT cells RNAseq dataset. Each sheet includes DE genes predicted by comparing RNAseq dataset from two timepoints.

Supplementary File 2 : Pathways enriched from differentially expressed genes in gdT cells RNAseq dataset, using pathfindR.

Supplementary File 3 : Differentially expressed genes predicted in Bulk RNAseq dataset. Each sheet includes DE genes predicted by comparing RNAseq dataset from two timepoints.

Supplementary Figure S1: (A) KEGG hsa04330 Notch signaling pathway, (B) KEGG hsa04540 Gap junction pathway, (C) KEGG hsa04662 B cell receptor signaling pathway, (D) KEGG hsa04350 TGF-b pathway. Transcripts identified to be differentially expressed in sort...