scRNAseq of spleen and atherosclerotic plaques from young and aged healthy and young and aged atherosclerotic mice

T cells change substantially with age and are involved in atherosclerosis. Aging is the strongest clinical risk factor for atherosclerosis so we profiled T cells in young and aged mice prior to atherosclerosis (healthy) and in young and aged atherosclerotic mice (diseased). Spleens from 3-month or 18-month and atherosclerotic plaques from 5-month and 21-month female C57BL/6 mice were rendered into single cell suspension, enriched for CD3+ cells and sequenced.