Gene expression profile at single cell level of cells isolated from the bone fracture gap and un-injured bone marrow in mouse osteotomy models

We investigated the cell composition in the bone fracture (osteotomy) gap under two different ambulatory loading conditions (stiif vs. compliant - external fixator). In addition, we applied an receptor inhibitor or respective isotype control to understand the role of a specific progenitor subpopulation during the onset of bone fracture repair. Un-injured bone marrow was used as control. Some samples were combined prior to library prep as indicated in the sample titles Overall design: Cells from the bone fracture (osteotomy) gap and contralateral bone marrow were isolated 3 days post-osteotomy. Erythrocyte lysis and live cells sorting was performed before scRNAseq. Female C57BL/6J mice (Jackson Laboratory) aged 14-16 weeks were used.