<i>KU70</i> and <i>KU80</i> genes in <i>P. tetraurelia</i>.

(A) Diagram of the WGD relationships between KU70 or KU80 genes in P. tetraurelia. The % of identity between genes (nt) and proteins (aa) are indicated along the arrows connecting two genes. (B) Transcription profiles of KU70 and KU80 genes during an autogamy time-course of strain 51, as determined by high-throughput RNA-seq. V: vegetative cells; S: starved or meiotic cells with intact parental MAC; T0: 50% of cells with fragmented MAC; the following time-points refer to hours after T0 [51]. On the vertical axis, FPKM represents the number of fragments per gene kb per million of fragments that were uniquely mapped on the genome. (C) Detection of KU70 and KU80c mRNA during autogamy, through northern blot hybridization. Control RNAi: RNAi against the nonessential ND7 gene [43], which encodes an exocytosis protein. V: vegetative cells. The times refer to hours after T0, the time at which 50% of cells have a fragmented MAC (Figure S1A).