RNA-seq of H2BmCh iOE mouse hearts

Cardiomyocyte nuclei are constantly exposed to mechanical stress. It remains mysterious how they maintain nuclear shape throughout a life. In this study, we found that high-level overexpression of H2B-mCherry in mouse cardiomyocytes causes elongation of nuclei with disrupted spatial organizations of heterochromatin. Cardiomyocyte nuclei in H2B-mCherry iOE mice show heterochromatin disruption at 2 weeks after TMX induction, leading to nuclear deformation, resulting in fatal heart failure at 6 weeks after TMX induction. The purpose of this RNA seq is to investigate the change of gene expression accompanied with heterochromatin disruption and nuclear deformation. We isolated heart tissue from H2B-mCherry iOE mouse hearts at 0 days, 2 weeks, and 6 weeks post-TMX, followed by RNA extraction, then subjected to RNA-seq.