Additional file 1 of ExonSurfer: a web-tool to design primers at exon–exon junctions

Additional file 1: Supplementary Table 1 displays primer designs generated using ExonSurfer, including gene details, efficiency percentages, tested cell lines, primer sequences, amplicon characteristics, melting temperatures, alignment scores, and transcript detection outcomes. Primer efficiency is calculated by the equation E = 10-1/slope, with an efficiency of 2 indicating that for each cycle the amount of product doubles.