Single cell RNA sequencing reveals heterogeneity and dynamics of mouse early inguinal white adipose tissue (iWAT) during development

In this study, we comprehensively compared the changes in adipose tissue from 1 week (7 d) to 4 weeks (28 d) postnatally. To analyze the different cell groups and how their amount and function vary along with developmental time, we collected iWAT from 7-, 14-, 18-, 21-, and 28-day-old mice and enzymatically disassociated the tissues into single cells and performed single-cell RNA sequencing. Overall design: To profile cells in an unbiased manner from iWAT considering the apparent alterations in phenotype and gene expression in mouse iWAT during early life, we collected iWAT from 7-, 14-, 18-, 21-, and 28-day-old mice and enzymatically disassociated the tissues into single cells. Stromal vascular cell fractions (SVFs) were separated from mature lipid-containing adipocytes by centrifugation and were subjected to single-cell RNA-seq.