Multiscale tomographic characterization of 3D bioprinted glioblastoma multiforme fo drug screening

The objective of this study is to evaluate the effectiveness of two drugs, namely Temozolomide (TMZ) and a new molecule (MV1035), on 3D bioprinted human Glioblastoma Multiforme (GBM) models by using multiscale synchrotron radiation imaging. We intend to use X-ray micro-tomography (X-PCI-µCT) to investigate the overall structure of 3D bioprinted models and the cell distribution within them and and nano-holotomography (XNH-CT) to examine in detail the local cell aggregation and organization. No methodologies are available in the laboratory today to evaluate the effects of selected drugs in these types of models without affecting the spatial and molecular composition of the Extra Cellular Matrix (ECM). Our hypothesis is that the surrounding ECM can be a key actor in GBM progression and in the drug efficacy. Synchrotron analysis represents the unique way to confirm our hypothesis