Paired blood-brain transcriptomes (GTEx v10)

Overview This dataset contains paired blood and brain RNA-sequencing data from adult human donors. Data were organized into multiple sub-directories and compressed into a ZIP archive. RNA-sequencing data were collected and quantified by the Genotype Tissue Expression (GTEx) Consortium. Tissue-specific gene quantifications were made publically availble (https://gtexportal.org/home/downloads/adult-gtex/bulk_tissue_expression). This is the most recent release of RNA-sequencing data from GTEx (version10). Methods Count data were filtered to retain genes with a minimum of 5 counts and 0.1 reads per kilobase per million mapped reads (RPKM) per tissue in at least 10 donors. Counts were converted to log-2 transcripts per million (TPM) values to adjust for sequencing depth and gene length. Quantile normalization was applied to adjust for between-donor variation in expression distributions. In each tissue, linear regression models were used to remove gene-expression variance attributed to confounding variables, including: age, sex, the top 3 genome-wide principal components of genotypes, PCR method, sequencing platform, ischemic time, RNA integrity number (RIN), and score on Hardy death scale. Additionally, three principal components representing deconvoluted abundances of circulating leukocytes were regressed out of the whole blood gene-expression data.