Data acquired from testing the effect of PAR1 inhibitors on Plasmodium falciparum-induced loss of endothelial cell barrier function

This dataset consists of 18 <b>.csv</b> files of data underlying the graphs and charts in figures 2-6 of the related publication (see final paragraph for details). <br>The files are as follows:<b>-Combined analysis data for fig 2_TEER_includes normalisation.csv</b><b>-Combined analysis data for fig5_TEER_includes normalisation.csv</b><b>-Combined analysis data for fig6_TEER_includes normalisation.csv</b><b>-Data for fig2A+B+C_TEER_EA cells+inhibitors+thrombin_3.csv</b><b>-Data for fig2C_TEER_EA cells+inhibitors+thrombin_1.csv</b><b>-Data for fig2C_TEER_EA cells+inhibitors+thrombin_2.csv</b><b>-Data for fig3_flow cytometry HBMEC.csv</b><b>-Data for fig4A_TEER_aPC+thrombin_all time points shown.csv</b><b>-Data for fig4B_TEER_RBC+IE+lysate_all time points shown.csv</b><b>-Data for fig5+6_TEER_lysate+thrombin_#04-2+#91-2.csv</b><b>-Data for fig5+6_TEER_lysate+thrombin_#91-1.csv</b><b>-Data for fig5+6_TEER_lysate+thrombin_E5555-1+#04-1.csv</b><b>-Data for fig5+6_TEER_lysate+thrombin_E5555-2.csv</b><b>-Data for fig5+6_TEER_lysate+thrombin_Vorapaxar-1.csv</b><b>-Data for fig5+6_TEER_lysate+thrombin_Vorapaxar-2.csv</b><b>-Data for fig6_TEER_lysate_Vorapaxar+E5555+#04+#91-1.csv</b><b>-Data for fig6_TEER_lysate_Vorapaxar+E5555+#04+#91-2.csv</b><b>-Data for fig6_TEER_lysate_Vorapaxar+E5555+#04+#91-3.csv</b><br>The related publication investigated the hypothesis that the Thrombin-PAR1 coagulation/inflammation axis plays a significant role in barrier loss in cerebral malaria (CM), and that adjunct treatments targeting this might alleviate mortality or post-CM neurological sequelae in CM. The authors used a co-culture of primary human brain microvascular endothelial cells (HBMEC) with <i>Plasmodium falciparum</i> material, recombinant PfEMP1 or lysates from infected erythrocytes (IE), and measured barrier function by Trans Endothelial Electrical Resistance (TEER). A selection of Protease-Activated Receptor-1 (PAR1) inhibitors was tested for their ability to reverse the <i>P. falciparum</i> and thrombin induced decrease in barrier function.<br>The TEER data is from an initial screen with 10 PAR1 inhibitors and the human umbilical vein endothelial cell line EA.hy926 followed by more detailed testing of 4 selected inhibitors and HBMEC. In addition, the effect of IE or IE lysate on EPCR expression on HBMEC was determined by flow cytometry.