gene profiling at single-cell level of the K562 cells with CRISPR-Cas9 KO of either HUB162 or HUB161

HUB162 (chr11:42,878,450-42,883,450, hg38) was identified as an essential locus in the quiescent region in the K562 cells, and HUB161 (chr11:42,873,450-42,878,450, hg38) as a nonessential one. We used scRNA-seq of 10x Genomics Chromium to study gene expression after CRISPR-Cas9 deletion of either HUB162 (Ess) or HUB161 (Noness) Overall design: K562 cells stably-expressing Cas9 (mCherry) were infected with pgRNA-expressing lentivirus (EGFP) and then cultured for 10 days. GFP+/mCherry+ cells were sorted and scRNA-seq was performed according to the procedures desrcibed in 10x Genomics chromium GEM Single Cell 3' RNA library manual (v3)