Summary of the mutant alleles isolated in the sma-9 suppressor screen.

Suppression was determined based on the number of M lineage-derived coelomocytes (CCs). sma-9(cc604) hermaphrodites have 0 M lineage-derived CCs, while wild-type worms have 2.ND: not determineda The worms scored are homozygous for sma-9(cc604) and homozygous for the suppressor indicated.b The worms scored are homozygous for sma-9(cc604) and heterozygous for the suppressor indicated.c Reported in [20].d Reported in [21].e Reported in [22].f Both jj65 and jj85 complemented dbl-1(wk70) and do not carry any molecular lesions in the dbl-1 coding or 5’ and 3’ regulatory regions.gjj58 might be a dominant sma-9 suppressor and was not characterized in this study.hjj68, jj80, jj81 and jj84 were not characterized in this study due to their low degree of suppression of the sma-9 M lineage phenotype.i Predicted based on likely splicing defect.j The predicted truncation at the amino acid level was calculated assuming that the large deletion does not affect the stability of the lon-2 message in jj61 animals. The deletion also deletes the majority of the aman-1 coding region, leaving only 338bp of the aman-1 first exon to be present. The Susm phenotype is due to the loss of lon-2 function because jj61 failed to complement lon-2(e678) for the Susm phenotype.k Aberrant transcripts were confirmed by RT-PCR and reported in [22].l Corresponding genes were identified via a combination of linkage analysis (linkage to cup-5(ar465) III, sma-9(cc604) X), snip-SNP mapping [86], and complementation tests based on the Susm phenotype, with the following alleles: dbl-1(wk70), sma-6(e1482), daf-4(m63), sma-2(e502), sma-3(e491), sma-4(e729), lon-2(e678), and lon-1(e185).m Corresponding genes were identified via WGS (see Materials and methods).n Corresponding genes were identified via SNP-WGS (see Materials and methods).o In addition to carrying a mutation in sma-4, jj70 appears to carry another sma-9 suppressing mutation that maps to LG V and fails to complement jj65 and jj85.p All molecular lesions were identified or confirmed by Sanger Sequencing.q A fragment containing 3kb of upstream sequences, the genomic coding region and 2kb of downstream sequences of sma-6 rescued the Susm phenotype of jj69.Summary of the mutant alleles isolated in the sma-9 suppressor screen.