Transcriptomic analysis of swim bladders of Chu’s croaker (Nibea coibor) fed with proline supplementation

Proline is a key substrate for collagen synthesis, we hypothesized that supplemented proline could enhance collagen deposition in swim bladder of the Chu’s croaker (Nibea coibor). Here, we obtained the fisrt transcriptome of the fish by next generation sequencing (NGS) and de novo assbemby. We annotated the unigene of the transcriptome and analyzed differentially expressed genes (DEGs) between swim bladder from fish fed without/with proline supplementation. Several important DEGs and KEGG pathway were identified to be potentially involved in the enhancement of collagen deposition by proline. File S1: Annotation of Unigenes in different databases; File S2: DEGs between swim bladder from fish fed without/with proline supplementation; File S3: Gene Ontology distribution of the unigenes and DEGs ； File S4: KEGG pathway enrichment analysis of the DEGs; File S5: KEGG pathway distribution of the DEGs.脯氨酸（Proline）是胶原蛋白合成的关键底物。我们推测，补充脯氨酸可增强黄姑鱼（Chu’s croaker，学名Nibea coibor）鳔中的胶原蛋白沉积。本研究通过下一代测序（next generation sequencing, NGS）与从头组装（de novo assembly）技术，首次获取该鱼类的转录组数据。随后，我们对该转录组的单基因（unigene）进行功能注释，并分析了投喂添加脯氨酸饲料与未投喂脯氨酸饲料的鱼的鳔组织间的差异表达基因（differentially expressed genes, DEGs）。最终鉴定出数个潜在参与脯氨酸促进胶原蛋白沉积过程的关键差异表达基因与KEGG通路。 附件S1：不同数据库中单基因的注释结果； 附件S2：投喂添加脯氨酸与未添加脯氨酸饲料的鱼的鳔组织间的差异表达基因； 附件S3：单基因及差异表达基因的基因本体（Gene Ontology, GO）分布情况； 附件S4：差异表达基因的KEGG通路富集分析结果； 附件S5：差异表达基因的KEGG通路分布情况。