Gene expression-based, individualized outcome prediction for exposure of rat ovarian tissue

Hierarchical clustering of the dysregulated genes from ovary of SD rat in different groups were successfully constructed through expression profiling，which suggested large sets of differentially regulated genes in rat ovaries in response to TBT or BPA exposure, and the combined group had more regulated genes. Though these differential expression genes, we can see the differentiation of agents of TBT and BPA. And according to these genes, we could farther study these related pathways to the PCOS. Using the selection criteria (FC ≥ 2, p ≤ 0.05), 86 up-regulated genes and 71 down-regulated genes were identified in the TBT100 group. In the BPA50 group, 45 up-regulated genes and 95 down-regulated genes were identified using the same criteria. The TBT+BPA group had 258 up-regulated genes and 234 down-regulated genes. There are three experimental groups and one control group. Each group has three sample replicates.From postnatal day 1 (PND1) to postnatal day 16 (PND16) , pups were injected TBT 100 ng/kg (TBT100), BPA 50μg/kg (BPA50) and TBT100 combined with BPA50 (TBT+BPA), which were administered by subcutaneous injection, whereas the control rats received an equal volume of vehicle (5 μL/g). All compounds were dissolved in ethanol and then sesame oil at a ratio of 10% ethanol and 90% oil. The vehicle was also prepared with this ratio. Actual dosing volumes were adjusted based on the body weights recorded every day. Please note that each raw data file contains raw data for two samples as indicated in the corresponding sample title and description field. For example, the 'Block_Column_Row' 1_1_1 to 1_272_194 (in the 1-1--2-1.gpr file) contains the first sample (i.e. [1-1]) raw data and the 'Block_Column_Row' 1_1_195 to 1_272_349 contains the second sample (i.e. [2-1]) raw data.