Reprogramming of epidermal keratinocytes by PITX1 transforms the cutaneous cellular landscape and promotes wound healing [scRNA-seq]

Skin injuries heal slowly, compared to oral epithelial tissues, and often do not regenerate lost adnexa. Using a murine mouse model expressing the oral epithelial transcription factor PITX1 in the skin, we performed scRNA-seq in healthy and wounded skin and healthy oral mucosa to delineate the cellular and molecular changes underpinning the superior oral wounding response. Overall design: Whole murine skin (healthy and POD4-wounded, Control and PITX1+) or buccal mucosa (healthy Control) was dissociated into single cells and analyzed via scRNA-seq.