Supplementary information files for Physico-chemical characterisation of Ti-Nb-Sn alloys surfaces and their osteogenic properties

Supplementary information files for Physico-chemical characterisation of Ti-Nb-Sn alloys surfaces and their osteogenic propertiesmplanted tissue engineering devices interact with the host tissue through their surface in the first instance. Surface chemistry triggers cell activities that stimulate bone tissue-formation mechanisms for osteoblast maturation. In this work, the bioactivity of binary Ti-40Nb and Ti-10Sn and ternary Ti-10Nb-5Sn alloys, candidates for bioengineering applications, has been studied on their surface with a view to establish their osteogenic potential compared to that of c.p. Ti. Cellular population growth was used to assess proliferative and differentiative phenotypes (via protein and Alkaline Phosphatase markers), coupled with gene expression (i.e. Runx2 and OCN) to confirm maturation. The results show that Sn-containing alloys support cell bioactivity, increase metabolic activity (i.e. metabolites content) that indicate their preferred glycolytic pathway, promote cell attachment, differentiation and osteoblast maturation. Ti-40Nb, although also non-cytotoxic, retards osteoblastic differentiation and maturation. To elucidate the features that underpin this difference, their physical (i.e. wettability, electrical state near the surface) and chemical properties (i.e. oxide layer thickness and composition) were analysed independently from topology and roughness. It was concluded that composition (esp. TiO2 % content) is a more important factor than wettability and oxide layer thickness, and that although a negatively-charged surface (represented by the surface ζ potential) was preferential for cell bioactivity given its protein-adsorption readiness, its magnitude was not a defining cause.

本数据集包含了用于表征Ti-Nb-Sn合金表面物理化学特性及其骨生成特性的补充信息文件。在组织工程植入器件与宿主组织首次接触时，其表面化学性质触发了细胞活动，进而刺激骨骼组织形成机制以促进成骨细胞的成熟。本研究中，针对生物工程应用候选材料二元Ti-40Nb和Ti-10Sn以及三元Ti-10Nb-5Sn合金的表面生物活性进行了研究，旨在评估其骨生成潜力与纯钛相比。通过细胞种群生长评估了增殖和分化表型（通过蛋白质和碱性磷酸酶标记物），并结合基因表达（即Runx2和OCN）以确认成熟。结果表明，含Sn合金支持细胞生物活性，增加代谢活性（即代谢物含量），表明其偏好糖酵解途径，促进细胞粘附、分化和成骨细胞成熟。尽管Ti-40Nb也非细胞毒性，但它会延缓成骨细胞的分化和成熟。为了阐明支撑这种差异的特征，对其物理性质（即润湿性、表面附近的电态）和化学性质（即氧化物层厚度和成分）进行了独立分析，而未考虑拓扑结构和粗糙度。结论是，成分（特别是TiO2含量百分比）相较于润湿性和氧化物层厚度更为重要，尽管带负电的表面（由表面ζ势表示）由于其蛋白质吸附准备性而优先于细胞生物活性，但其幅度并非决定性因素。