MSC differentiation in adipocytes, osteocytes and chondrocytes.
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(A) Cultured MSC have fibroblastoid morphology under phase contrast; inset shows adherent MSC in higher magnification. (B) Cells were cultivated in adipogenic medium, fixed and incubated with Oil Red O to identify lipid vacuoles (red staining). (C) Cells were cultured in osteogenic medium and calcium deposits were revealed with alizarin red (yellowish-brown staining). (D) Cells were cultivated in chondrogenic medium, forming a micromass; proteoglycans were stained with alcian blue and nuclei counterstained with Nuclear Fast Red. Scale bar: 50 µm (A), 12.5 µm (B,C), 50 µm (D).
创建时间：
2016-02-23
