GRR pattern in Ago2 in different <i>D. melanogaster</i> strains.

Genomic DNA encompassing the GRR1 or GRR2 repeats was amplified using PCR. The number of repeats was estimated by gel electrophoresis as in Fig. 3A (numbers in brackets) or determined by sequencing the PCR fragments (Fig. S1). Top: The 3CPA strains were derived from a single wild North American population in 2001 [32]. Middle: Wild-type laboratory strains; note that two strains characterized as Or R (obtained from different laboratories) had distinct GRR repeat patterns. Bottom: A selection of other laboratory strains; homozygous lethal chromosomes (LVM, TM6B, TM1, Dp(3;3)Cam36) were analyzed as transheterozygotes with the deficiency Df(3L)XG9 that encompasses the ago2 locus.