Raw data for: "Comprehensive analyses of bulk and single-cell transcriptomes identify ammonia-induced biomarkers associated with cell death and immune microenvironment remodeling in Alzheimer's disease"

This dataset contains the raw wet experimental data and bioinformatics analysis code associated with the manuscript entitled "Comprehensive analyses of bulk and single-cell transcriptomes identify ammonia-induced biomarkers associated with cell death and immune microenvironment remodeling in Alzheimer's disease".### Research BackgroundAlzheimer's disease (AD) is a progressive neurodegenerative disorder, and ammonia-induced cell death (ACD) has been implicated in AD pathogenesis. This study integrates bulk RNA-seq, single-cell RNA-seq, machine learning, and in vitro experiments to identify ACD-associated biomarkers and explore their roles in AD-related immune microenvironment remodeling.### Data Content1. **Wet experimental data**: - TUNEL staining images (original uncropped files and cropped representative figures for manuscript visualization) - qPCR raw Ct values and quantification results - Western blot original band images and grayscale quantification data - Cell viability/apoptosis assay data (CCK-8, flow cytometry)2. **Bioinformatics code**: - Scripts for bulk/single-cell RNA-seq data processing, differential expression analysis, and functional enrichment - Machine learning code for biomarker screening - Code for immune cell infiltration analysis and data visualization### Image Processing NoteAll representative microscopy images in the manuscript have been cropped to improve visual clarity and focus on representative fields. No data manipulation, signal enhancement, or removal of experimental results was performed; all quantitative analyses were based on the full, uncropped original images.