Fluorescent Membrane Tension Probes for Early Endosomes

<strong>Table of contents</strong> 1. Synthesis and Characterization 1.1. Compounds <strong>1</strong>,<strong> 3</strong>,<strong> 4</strong>,<strong> 6</strong>,<strong> 7</strong>,<strong> 9</strong>,<strong> 10</strong>, <strong>11</strong>,<strong> 12</strong> Lab Book Page NMR LC-MS IR HRMS 1.2. Compounds <strong>15</strong>,<strong> 17</strong>,<strong> 19</strong>,<strong> 24</strong>,<strong> 1’</strong>,<strong> 3’</strong>,<strong> 5’</strong>,<strong> 7’</strong> Lab Book Page NMR LC-MS IR ESI-MS 1.3. Compounds <strong>21</strong>,<strong> 23</strong>,<strong> 26</strong>,<strong> 8’</strong>,<strong> 10’</strong> Lab Book Page NMR LC-MS IR 2. p<em>K</em>_a Determination 2.1. Titration of <strong>32</strong>,<strong> 35</strong>,<strong> 36</strong> for the determination of the correction function Lab Book Page NMR 2.2. Titration of Model Compounds <strong>1’</strong> – <strong>10’</strong> Lab Book Page NMR 3. Co-Localization with EGF 4. Automated Microscopy 5. FLIM Imaging 6. FLIM of Early Endosomes