Proteome-wide characterization of FAS2 genomic elements swap

Protein synthesis is a highly regulated process that ensures the efficient assembly of protein complexes. Co-translational interactions are crucial to ensure the timely formation of functional protein complexes. One factor that can facilitate complex assembly is the spatial organization of mRNA within the cell. In this study we investigate the spatial distribution of mRNAs encoding for protein complex subunits in Saccharomyces cerevisiae, focusing on determining the co-localization state of these mRNAs and its impact on protein assembly and functionality. By swapping cis-regulatory elements upstream and downstream of the coding sequence we identified patterns that influence mRNA co-localization. We further quantified the resulting changes in global protein abundance in the swapped strains using LC-MS.